he NMR facility, Room 40, is a state of the art facility with three Varian NMR spectrometers, Varian-500S, Mercury-400, and Unity-300. The Department also has a Bruker 700 MHz. instrument that runs in parallel with CIF operations. The NMR laboratory offers to the Department of Chemistry and the university community (graduate students, Postdocs and Faculty members) with ample time availability, supreme technical and training support, and responsive NMR services with the state of the art versatile and friendly software to perform the most sophisticated 1D and 2D NMR experiments in the most time efficient protocol for users.
NMR Instrumentation
The NMR facility has three Varian spectrometers; Unity-300 is a multinuclear dual-band system with fixed proton frequency channel and equipped with three 5 mm probes: The standard probe for this system is Four-Nucleus (13C, 31P/1H, 19F) with Variable temperature capabilities. Two 5 mm Dual broadband (15N-31P/1H-19F) probes are available for multinuclear observation. The Hardware and software options on this instrument provide adequate support for 15-25 mg samples to obtain routine NMR data (1H, 13C, and DEPT) in less that fifteen minutes. Variable temperature experiments are used very extensively on this instrument. Most correlation 2D NMR experiments are available as a standard routine for all users.
Varian Mercury-400 spectrometer is our work horse for most of our NMR users for complete structure characterization. The instrument is a multinuclear broadband with Pulsed Field Gradient and variable temperatures capacity. Two 5 mm probes are available for multinuclear observation and PFG applications; the standard probe is Four-Nucleus (13C, 31P/1H, 19F) with Dual broadband (15N-31P/1H-19F) and PFG capabilities, the second probe is Dual broadband (15N-31P/1H-19F) which is used for multinuclear observation, and variable temperature, and as a back up tool for the system. All 1D (1H, 13C, DEPT, Homodecoupling, and Selective NOE) and Inverse detection, PFG 2D NMR (GCOSY, GHMQC, GHSQC, and GHMBC) experiments are performed regularly on 10-15 mg samples in less than fifteen minutes.
Varian-500S spectrometer with new seal for Oxford magnet and installed with new Varian console (December, 2006). The new system is our state of the art research spectrometer equipped with new Varian technology incorporating the sophisticated DirectDrive™ architecture and designed for unsurpassed flexibility with new AutoX probe and VNMRJ software. Due to the higher sensitivity and increased dispersion for dilute and demanding samples (organic and biomolecules). The new console is multinuclear dual broadband system, Pulsed Field Gradient and variable temperature capabilities. The two channels have pulse shaping hardware and software to perform sophisticated and complex multipulse NMR sequences for 1D, 2D, and 3D NMR experiments. We have four probes available for multinuclear observation and X-decoupling. The standard probe is 5mm AutoX Dual broadband (15N-31P/1H-19F) with variable temperature and PFG capabilities. The second probe is 5mm dual band (15N-31P/1H-19F) with PFG and variable temperature capabilities and Used as a backup for the standard probe . The third probe is 5mm inverse detection 1H-19F{X} used for higher proton sensitivity and X-decoupling experiments. The fourth probe is triple Nuclei 1H {15N, 13C, 31P} with PFG and variable temperature capabilities. This probe is designed for biomolecule applications for higher sensitivity and the availability of two channels X-decoupling with PFG 2D and 3D NMR. Experiments. The 5 mm standard AutoX probe is utilized 70% of the available time for all 1D (1H, 13C, DEPT, Homo-decoupling, and Selective NOE) and Inverse detection, PFG 2D NMR (GCOSY, GHMQC, GHSQC, and GHMBC) experiments are performed regularly on 5-10 mg samples in less than fifteen minutes.
All Varian NMR spectrometers are equipped with similar user friendly in house programmed software for ease of operation and optimum working spectrometer conditions for time saving operation.
The NMR facility provides technical and NMR lecture classes twice during the academic year (30 lecture hours to explore over 30 NMR experiments) at all levels.
Cool video of the 500 MHz quenching (WMV format) Hi Res 11MB || Lo Res 1.3Mb
