Wayne State University

Aim Higher



College of Liberal Arts & Sciences
Department of Chemistry
Faculty Page
 
Young-Hoon Ahn
Title Assistant Professor
Division Biochemistry
Education B.S., POSTECH (Pohang University of Science and Technology), 1999
M.S., POSTECH (Pohang University of Science and Technology), 2001
Ph.D., New York University, 2007
Postdoctoral Fellow, Johns Hopkins University School of Medicine, 2008-2012
Office Chem 421
Phone (313)577-1384
E-Mail
Group http://chem.wayne.edu/ahngroup


The research interest in my laboratory lies in the field of chemical biology. Our goals are 1) to understand the significance of oxidative protein modifications that occur in redox signaling or oxidative stress and 2) to develop small molecules that can provide cellular protection against oxidative damages. To this end, we take multidisciplinary approach at the interface of chemistry and biology, including synthetic organic chemistry, protein biochemistry, and cell biology.

It is increasingly recognized that the reactive oxygen and nitrogen species (ROS/RNS) can be transiently generated in physiological conditions in response to various stimuli and they act as signaling molecules in redox signaling that regulates diverse cellular processes. In such condition, ROS/RNS are often sensed by unique redox chemistry of thiol in a protein cysteine residue that undergoes various types of oxidative modifications, such as S-glutathionylation, sulfenylation, S-nitrosylation, and disulfide bond formation. Importantly, these oxidative cysteine modifications profoundly influence physiological functions of many proteins, serving as a regulatory switch of many cellular processes. Consequently, its aberrant regulation often leads to oxidative stress, imbalance between the generation and the removal of ROS/RNS, which correlates with many disease conditions, including diabetes, cancer, cardiovascular and neurodegenerative disease.

Chemical Tools to Understand Oxidative Protein Modification
We are interested in developing chemical tools that lead to understanding oxidative protein modifications in response to ROS/RNS. We are applying these tools to analyze such protein modifications in disease-simulated cell-based system.

Site-specific Oxidative Protein Modification
We are also interested in understanding functional significance of oxidative protein modifications. To this end, we use semi-synthetic protein synthesis to introduce the site-specific oxidative protein modification, and evaluate structural and functional alterations by various biochemical, biophysical methods and cell-based systems.

Targeting Cytoprotective Pathways
We explore two important signaling pathways that can provide cellular protection against oxidative damages: namely, anti-oxidant response and heat shock response regulated by two transcription factors, Nrf2 (nuclear factor E2-related factor 2) and Hsf1 (heat shock factor 1), respectively. Many animal/human model studies indicate that activation of these pathways is an effective strategy of conferring protection against disease conditions that involve oxidative stress and/or protein misfolding, such as cancer and neurodegeneration. We are developing small molecules that selectively regulate these pathways by use of various chemical approaches, including small molecule screening and fluorescent assay.





REPRESENTATIVE PUBLICATIONS

Samarasinghe, K. T. G., Munkanatta Godage, D. N. P., Zhou, Y., Ndombera, F. T., Weerapana, E., Ahn, Y. H.* Clickable Glutathione Approach for Identification of Protein Glutathionylation in Response to Glucose Metabolism. Mol. Biosyst. 2016, In Press.

Ndombera, F. T., VanHecke, G. C., Nagi, S., Ahn, Y. H.* Carbohydrate-based Inducers of Cellular Stress for Targeting Cancer Cells. Bioorg. Med. Chem. Lett. 2016, 26, 1452-1456.

Samarasinghe, K. T. G., Ahn, Y. H.* Synthesizing Clickable Glutathione by Glutathione Synthetase Mutant for Detecting Protein Glutathionylation. SynLett. 2015, 26, 285-293.

Samarasinghe, K. T. G., Munkanatta Godage, D. N. P., VanHecke, G. C., Ahn, Y. H.* Metabolic Synthesis of Clickable Glutathione for Chemoselective Detection of Glutathionylation. J. Am. Chem. Soc. 2014, 136, 11566-11569.

Zhang, Y., Naidu, S. D., Samarasinghe, K. T. G., VanHecke, G. C., Pheely, A., Boronina, T. N., Cole, R. N., Benjamin, I. B., Cole, P. A., Ahn, Y. H.*, Dinkova-Kostova, A. T.* Inhibition of HSP90 by sulfhydryl-reactive sulfoxythiocarbamates. Brit. J. Cancer. 2014, 110, 71-82.

DEPARTMENT OF CHEMISTRY ©
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5101 Cass Ave, Detroit, MI 48202
Phone: (313) 577-7784    Fax: (313) 577-8822

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